A Cell Free Assay to Study Chromatin Decondensation at the End of MitosisAnna K. Schellhaus *1, Adriana Magalska *2, Allana Schooley 1, Wolfram Antonin 1
1Friedrich Miescher Laboratory, Max Planck Society, 2Nencki Institute of Experimental Biology, Polish Academy of Sciences
The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.
